Antibiotic prophylaxis for transrectal ultrasound-guided prostatic biopsies: a comparison of two regimens
African Journal of Urology volume 26, Article number: 11 (2020)
There were controversies about the appropriate antibiotic prophylaxis regimen in order to decrease the incidence of post-transrectal ultrasound-guided prostatic biopsies (TRUS-PB) infectious complications. This study was conducted to compare the efficacy of two antibiotics prophylaxis regimens for TRUS-PB. In group 1, patients received single IV dose of 1 g amikacin 30 min before the procedure followed by oral ciprofloxacin 500 mg immediately after TRUS-PB. Group 2 patients received the same antibiotics, but ciprofloxacin started 1 day prior to TRUS-PB. Then ciprofloxacin was given twice daily for a total of 5 days in both groups.
The study included 146 patients (54 in group 1 and 92 in group 2). The baseline characters (age, comorbidities, ASA score, PSA, prostate size and presence of urethral catheter) were comparable for both groups. Post-biopsy sepsis was observed in three patients (5.6%) in group 1 and one patient in group 2 (1.1%, P = 0.143). Sepsis was successfully managed in three, while one patient (1.9%) from group 1 required ICU admission for management of septic shock.
The incidence of sepsis after TRUS-PB is low when dual antibiotic prophylaxis (ciprofloxacin and amikacin) was used. Starting ciprofloxacin 1 day before TRUS-PB decreased the incidence of sepsis as well as its severity.
Prostate cancer is the second most commonly diagnosed cancer in men after cutaneous cancers . Prostatic needle biopsy is currently the standard technique to obtain tissue for histological diagnosis of prostate cancer. Recent studies have showed that approximately one million prostatic biopsies are performed per year in the USA . TRUS-guided prostate biopsies (TRUS-PB) technique is considered to be a relatively low-risk outpatient procedure with some minor self-limiting complications such as rectal bleeding and hematospermia . However, major complications necessitating emergency department visits and hospital admissions such as sepsis, urinary retention and gross hematuria were also reported in a minority of patients .
Post-TRUS-PB hospital admissions have escalated at alarming rates during the last 10 years secondary to the rising rate of infection-related complications . The European Association of Urology (EAU) guidelines for antibacterial prophylaxis for TRUS-PB recommend fluoroquinolones as a first choice due to their broad spectrum of activity, excellent penetration into prostatic tissue, and their prolonged post-antibiotic effect . As a consequence of being the current prophylaxis of choice for most urologic procedures, the wide spread of fluoroquinolone has led to colonization of rectal flora with fluoroquinolone resistant E. coli in 23% of patients undergoing prostate biopsy . This resulted in increase in the number of post-TRUS-PB infections and sepsis in the last few years, and most cases of sepsis in the recent literature are triggered by fluoroquinolone-resistant E. coli .
There were controversies about the appropriate antibiotic prophylaxis regimen in order to decrease the incidence of infectious complications. Joel et al. surveyed 3355 urologists in USA and reported 14 different durations of treatment using 10 different classes of antibiotic . Timing for start of the antibiotic was also different as some proved giving the antibiotic immediately after prostate biopsy was as effective as giving it 30 min  or 2 h before the biopsies . On the other hand, it was proved that giving the antibiotic 24 h before prostatic biopsies reduced relative risk of infection by 55% compared with antibiotics given just prior to the procedure .
We conducted this study to compare the efficacy of two antibiotics prophylaxis regimens for TRUS-PB.
The archived files for patients who underwent TRUS-PB between January 2012 and August 2018 were retrospectively reviewed after IRB approval of the study protocol. Pre-biopsy evaluation included history, digital rectal examination (DRE), PSA, INR and urine culture. Inclusions criteria were abnormalities in DRE and/or high PSA in relation to patients’ age. Patients with coagulopathies or receiving corticosteroids or immune suppression were not included. Those with active UTI (positive urine culture) received the specific antibiotic, and the procedure was postponed until urine culture became sterile. Patients receiving antiplatelets stopped them (after consultation of the treating doctor) for sufficient time before the biopsies.
2.1 Antibiotic prophylaxis protocols
Patients who underwent TRUS-PB between January 2012 and April 2015 represent group 1. They received single IV dose of 1 g Amikacin 30 min prior to the procedure then oral ciprofloxacin 500 mg immediately after TRUS-PB for 5 days. Group 2 included patients who underwent TRUS-PB between May 2015 and August 2018. In addition to the single IV dose of 1 g amikacin, they received oral ciprofloxacin 500 mg 1 day before the biopsy and continued twice daily for a total of 5 days.
2.2 Technique of TRUS-PB
All patients received bowel preparation one night before the procedure (Picolax®, Ferring pharmaceutical, UK). The same biopsy technique was performed by two urologists (MF and SMS) who are experienced in TRUS-PB for all patients. In a left lateral position after applying local anesthetic (Xylocain gel), a Voluson® E6 machine (GE medical system, Milwaukee, USA) was used, with a side-firing probe; Voluson® Endocavity transducer. The prostate was scanned from its apex to base, and the prostatic volume was calculated. A systematic 12-core biopsies were taken for all patients using automatic disposable biopsy instrument and an 18-gauge (Monopty®, BARD®, Tempe, USA). Additional 1–2 biopsies were obtained from any suspicious area in the prostate (hard nodule by DRE or hypoechoic lesion on TRUS).
2.3 Outcome evaluation
Primary outcome was incidence of post-TRUS-PB sepsis necessitating hospital admission. Sepsis was documented in the presence of two or more of fever ≥ 38 °C, leukocytosis (< 12,000/mm3) and tachycardia < 90/min. Septic shock included the previous in addition to hypotension. Post-biopsy complications were graded according to modified Clavien classification .
2.4 Statistical analysis
Statistical analysis was performed using SPSS v20 Program. Categorical data were expressed as numbers and percentages and then compared between groups using Chi-square test or Fisher’s exact test. Continuous data were expressed as mean and standard deviations (SD) or median and interquartile range (IQR) if the values were not normally distributed and then compared using t test or Mann–Whitney U test. P value < 0.05 was considered significant.
The study included 146 patients (group 1 included 54 patients and group 2 included 92 patients). Mean age was 64 years (SD 8.7). Median PSA level was 8.9 ng/ml (IQR 6–15.6). The baseline characters were comparable for both groups (Table 1). Prostate cancer was detected in 56 patients (38.5%).
The overall incidence of post-TRUS-PB sepsis was 2.7% (4 out of 146 patients). It was observed in three patients in group 1 (5.6%) and one patient in group 2 (1.1%), but the difference was insignificant (P = 0.143). Sepsis developed within 48 h after TRUS-PB, and E. coli was detected from blood culture in the four patients. Antibiotic sensitivity showed resistance to ciprofloxacin in two patients. Sepsis was successfully managed with IV antibiotics, fluids and antipyretics for three patients (Grade 2 complication), while one patient (1.9%) from group 1 required ICU admission for management of septic shock (Grade 4a complications). Post-biopsy urinary retention developed in one patient from group 2 and was catheterized for 5 days (Grade 3a complication).
Sepsis and septic shock are major complications of TRUS-PB because they require hospital admission, urgent treatment and sometimes ICU admission. The incidence of post-TRUS-PB sepsis ranged from 0.17 to 5.7% [13,14,15]. The overall incidence of sepsis in the present study (2.7%) was within the published range of previous reports [13,14,15]. More sepsis (5.6%) was observed in group 1 (patients who started ciprofloxacin after prostatic biopsies) in comparison with group 2 (1.1%) (those who started ciprofloxacin 1 day before the biopsies). This difference was not statistically significant but starting oral antibiotic after the biopsies was associated with 1.9% incidence of septic shock, which is more than previously reported range of 0.2–0.45% [13, 14]. On the other hand, antibiotic administration prior to biopsies was not associated with septic shock in all patients in group 2.
There is no single accepted protocol for antibiotic prophylaxis for TRUS-PB with regard to antibiotic class, number, timing of start and duration . In most of the recent protocols, two antibiotics were used (a fluoroquinolone and an aminoglycoside) [15,16,17] or a specific antibiotic based on rectal swab culture and sensitivity results [7, 15, 18]. The emerging need for this was the escalating incidence of fluoroquinolone resistant bacteria in rectal flora to reach 22% in one study  and up to 83% in another report . A combination of ciprofloxacin and amikacin was used in the current study to combat fluoroquinolone resistant bacteria. It was reported by Kehinde et al.  that adding amikacin to ciprofloxacin significantly decreased the rate of TRUS-PB sepsis. Yang et al. , in their recent meta-analysis, concluded that adding one antibiotic to the basic agent may contribute to minimizing infection severity. This was proved in the present study as no patient in group 2 developed septic shock. Elshal et al.  had found that two antibiotics administration was associated with lesser incidence of post-TRUS-PB infectious complications than rectal swab-based prophylaxis. Steensels et al.  had found that the use of fluoroquinolones in the previous 6 months before TRUS-PB was a risk factor for fluoroquinolone-resistant E. coli. Therefore, patients who received fluoroquinolones within 6 months prior to TRUS-PB can benefit from rectal swab-based prophylaxis.
In the first three consecutive years of the present study, ciprofloxacin was started immediately after TRUS-PB based on two previous studies that showed no differences in infectious complications between those who started antibiotic before or immediately after TRUS-PB [9, 10]. In the last month of this period, two cases of post-biopsy sepsis were encountered and one of them developed septic shock. This created the need to reconsider the antibiotic prophylaxis regimen adopted. Since then we started ciprofloxacin 1 day before TRUS-PB. This protocol resulted in reducing the incidence of sepsis and avoidance of septic shock. Manecksha et al.  also reported that starting antibiotic 24 h before TRUS-PB was associated with 55% reduction in the relative risk of infectious complications.
Duration of antibiotic prophylaxis was also a controversial issue as some authors used single dose of antibiotic [9, 10], others used it for 3 days [11, 17] or 5 days . In a meta-analysis of 22 randomized controlled trials, Yang et al. had found twofold of greater risk of bacteriuria for short-course treatment and single-dose treatment when compared with long-course. They also concluded that using two antibiotics for long-course can decrease development of drug resistance . In the present study, we administered ciprofloxacin for 5 days to achieve the value of lesser post-biopsy infections and minimize the chances of drug resistance.
The antibiotic prophylaxis protocols and incidence of sepsis after TRUS-PB in contemporary reports are summarized in Table 2. The highest incidence of post-TRUS-PB sepsis was observed in patients who received ciprofloxacin alone [17, 20], while the lowest incidence was reported with antibiotic prophylaxis depending on rectal swab cultures .
One of the limitations of this study is retrospective design that affected our ability to report minor complications such as rectal bleeding, mild hematuria and hemospermia because the data about these complications were incomplete. Another limitation is the relatively small sample size in group 1. Therefore, multivariate analysis for risk factors was not performed.
The incidence of sepsis after TRUS-PB is low when dual antibiotic prophylaxis (ciprofloxacin and amikacin) was used. Starting oral ciprofloxacin 1 day prior to TRUS-PB decreased the incidence of sepsis as well as its severity.
transrectal ultrasound-guided prostatic biopsies
American Society of Anesthesia
prostatic specific antigen
European Association of Urology
digital rectal examination
international normalization ratio
urinary tract infection
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MF was involved in data acquisition, SMS revised the manuscript, ARE was involved in conception, data analysis and drafting the manuscript, TFA revised the manuscript, and AA designed the work. All authors have read and approved the final manuscript.
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Ethics approval and consent to participate: (IRB number: 2018-18)
All procedures performed in this study were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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Faty, M., Saleh, S.M., El-Nahas, A.R. et al. Antibiotic prophylaxis for transrectal ultrasound-guided prostatic biopsies: a comparison of two regimens. Afr J Urol 26, 11 (2020). https://doi.org/10.1186/s12301-020-00026-9