The prognostic value of histopathological pattern of the pelviureteric junction in the outcome of pyeloplasty in children

Abstract

Background

The cause of pyeloplasty failure remains unclear; therefore, increasing interest has been directed to identify the reason for dissatisfactory surgical results. Some studies attempted to investigate the role of the histopathological pattern. The aim of the work is to study the correlation between the histopathological pattern of obstructed pelviureteric junction (PUJ) segment and the outcome of pyeloplasty in children with the PUJ.

Methods

Fifty-four patients with pelviureteric junction obstruction (PUJO) were included in the study; patients were evaluated preoperative. All patients were operated by the same surgeon using Anderson–Hynes pyeloplasty; the resected obstructed PUJ segment examined histopathological using light microscope and image analyzer system to identify mean renal pelvis smooth muscle thickness (mRPSMT), collagen-to-smooth muscle ratio and elastin content.

Results

The study includes 50 patients with mean age Mean 24.48 ± 15.3 months. Two patients show no improvement, while 24 improved at 3 months, 16 improved at 6 months, and 8 improved at 12 months. mRPSMT showed significant difference between improvement groups (136.02 ± 44.4, 173.47 ± 49.69 and 258.56 ± 96.82 μm), while elastin content or collagen-to-smooth muscle ratio showed no significant difference.

Conclusions

Our data showed a clear relationship between mRPSMT and the time over which radiological improvement occurs; increase in mRPSMT is associated with a delay in postoperative radiological improvement time. We found no relation between elastin content nor collagen-to-smooth muscle thickness and postoperative improvement course.

Background

Hydronephrosis caused by congenital pelviureteric junction obstruction (PUJO) is the most common urinary tract anomaly in childhood [1], affecting 1/1000–1/2000 of newborns [2]. The incidence of PUJO diagnosis is expected to be gradually increased secondary to the widespread use of maternal ultrasound [3]. Until recently, the underlying mechanism of PUJO is still unclear. Smooth muscle discontinuity or disproportionate presence of longitudinal smooth muscle fibers, together with excessive collagen deposition, has been suggested to be associated with the pathogenesis of congenital PUJO [4, 5].

Open dismembered pyeloplasty “Anderson–Hynes technique” has been considered the gold standard for treatment of PUJO for the last 70 years [6]. This technique was reported to be successful in a greater than 95% of patients. However, the remaining 5% still suffer from failure of procedures and the need for additional interventions to treat persistent obstruction [7, 8]. On the other hand, the exact time to evaluate postoperative improvement using radiographic examinations is still unexpected, resulting in long-term, difficult and subjective clinical follow-up of patients [9].

Several different causes are known to be related to initially unresolved hydronephrosis, including irreversible histopathological changes in the renal pelvis and caliceal collecting system, transient obstruction due to edema at the site of repair and persistent obstruction due to failed pyeloplasty [3]. Unfortunately, the cause of pyeloplasty failure remains unclear; therefore, increasing interest has been directed to identify the reason for dissatisfactory surgical results. Some studies attempted to investigate the role of the histopathological pattern of the PUJ in this failure; however, its role is still controversial [10]. So, we attempted to conduct a prospective study to investigate the effect of the histopathological pattern of the ureteropelvic junction on the outcome of pyeloplasty in children with PUJO.

Methods

This is a prospective single arm study of patients submitted to pyeloplasty due to PUJO at the department of Urology, between January 2016 and June 2018. The study was approved by the local ethical committee of our university. All patients’ parents provided written consent before participating in any study-related procedures.

The target population included patients diagnosed with PUJO. All patients with recurrent PUJO, any other urinary tract pathologies, active urinary tract infection (UTI), upper urinary tract congenital anomalies and vesicoureteric reflux were excluded.

Preoperative patient evaluation was performed including previous history, routine preoperative laboratory, renal ultrasound, intravenous urography, diuretic renogram and computerized tomography for urinary tract with contrast if indicated. All operations were performed by the same surgeon. All patients were operated using Anderson–Hynes pyeloplasty (dismembered pyeloplasty) [11], indwelling double-pigtail stent was placed for 6 weeks postoperative, and a drain was placed in the perinephric space. Histopathological specimen was obtained from obstructed PUJ site.

Staining method

All pathological assessments were examined at the department of pathology; specimens obtained for histopathological examination were fixed in 10% formalin. After fixation, the cleaned tissues underwent dehydration and buffering, and were embedded in paraffin. Blocks were cut into 4 μm transverse serial whole amount section. In order to identify collagen and smooth muscle, sections were stained with hematoxylin and eosin stain and sirius red stain (Figs. 1, 2). Moreover, to identify elastin, sections were stained with Van Gieson's stain (Fig. 3). Finally, stained sections were examined under a light microscope and image analyzer system to determine the histopathological data including collagen-to-smooth muscle ratio, mean renal pelvis smooth muscle thickness and elastin content. By using image analyzer system which is computer software capable of obtaining quantitative data from histopathological sections of images, simply we determine the particular shape of the wanted parameter (elastin, collagen, smooth muscle); then, we ask the software to calculate the wanted data (percentage, ratio and mean thickness) and then give us the data in numerical form not visual figures.

Fig. 1

Basophilic fibrocollagenous bundles in eosinophilic background “HX&Eosin × 100”

Fig. 2

Dark red-stained collagenous bundles in pink fibrohyaline background “syrus red × 100”

Fig. 3

Positive dark brown fibers of elastic tissue in eosinophilic background of fibrocollagenous bundles “×200-van gieson stain”; C collagen, E elastin

Parameters estimated

Age, gender, side of the lesion, time and results of postoperative radiographic examination, and time of definite improvement were identified in our study. All patients underwent a postoperative follow-up with a renal ultrasound (U/S) at the third month, the unresolved patients at sixth month and at twelfth month. The degree of improvement was defined as the difference between the preoperative and postoperative grades of hydronephrosis according to society of fetal urology (SFU) grading system. Diuretic renogram was used in selected cases when evaluation of prognosis by U/S could not be achieved.

Statistical analysis

Statistical analysis was performed using Statistical Package for the Social Science (SPSS Inc, Chicago, Illinois, USA) version 21.0. Patient characteristics were descriptively analyzed.

Results

Fifty-four patients were subjected to the study, but four patients missed from follow-up so they were excluded from the study.

So, 50 patients were assessed, and a total of 26 male and 24 female patients were identified (male to female ratio 1.08:1). The right-sided lesion was 26, while left-sided lesion was 24 with ratio 1.08:1, mean BMI: body mass index was 22.96 ± 3.36 (kg/m²) ranging from 20 to 35, mean age at operation was 24.48 ± 15.3 months with the youngest patient was 4 months and the oldest was 62 months, and the mean operative time was 89.4 ± 23.4 min.

According to SFU (society of fetal urology), there were 27 patients presented with grade III hydronephrosis, while 23 patients presented with grade IV hydronephrosis. Only two patients showed deterioration after removal of JJ stent, in the form of persistence of obstruction with increase in the hydronephrosis grade compared to the preoperative one, re-insertion of JJ stent failed, and the patients required re-operation (Table 1).

Table 1 Distribution of the studied group according to personal and histopathological data

The rest of the patients showed improvement but varying in the time over which the improvement happened, and the improvement time ranged from 3 to 12 months.

Patients who had grade III hydronephrosis improved to grade II hydronephrosis, with 14 patients improved at 3 months, eight patients improved at 6 months, and four patients improved at 12 months. While patients with grade IV hydronephrosis improved to grade III; ten patients improved at 3 months, eight patients improved at 6 months, and four patients improved at 12 months (Tables 2, 3).

Table 2 Grades of hydronephrosis preoperative and time of improvement

Table 3 Number of patients improved after different follow-up period

After statistical analysis, 24 patients improved at 3 months and had mRPSMT: mean renal pelvis smooth muscle thickness 136.02 ± 44.4 (μm), their collagen/smooth muscle ratio 1.32 ± 0.19, and their elastin content was 17.13 ± 3.13 (μm), while 26 patient still with no improvement, 16 patients out of 26 improved at 6 months and had mRPSMT 173.47 ± 49.69 (μm) their collagen/smooth muscle ratio 1.31 ± 0.23, and their elastin content was 17.81 ± 2.64 (μm), while 10 patients still showed no improvement, after 12 months 8 patients out of 10 showed improvement with their mRPSMT was 258.56 ± 96 (μm) their collagen/smooth muscle ratio 1.38 ± 0.09 and their elastin content was 18.25 ± 2.82 (μm), and 2 patients with no improvement at 12 months, we found correlation between mRPSMT and postoperative time of improvement but no correlation between elastin percentage and collagen-to-smooth muscle ratio to postoperative time of improvement (Table 4).

Table 4 mRPSMT, collagen/smooth muscle and elastin after different follow-up periods

Discussion

Since first described by Anderson and Hynes in 1949, open dismembered pyeloplasty remains the gold standard for PUJO treatment [11]. In more than 95% of cases, this technique is successful [12]. In spite of this very high success rate, the period over which satisfactory non-obstructive pattern of renal function is usually not predictable. In some cases, the initial postoperative radiological investigation shows no signs of successful surgical correction. This is extremely stressful situation for both surgeon and patients (or their parents); however, most of these cases show spontaneous improvement by time. Thus, finding predictor of the outcome course is urgently needed.

Histological changes in the renal pelvis in response to obstruction determine its compliance after pyeloplasty [1, 3]. Many studies—including our study—tried to correlate the outcome of the surgery with histopathological changes in the renal pelvis. Most of them focused on mRPSMT, collagen-to-smooth muscle ratio and elastin content of the renal pelvis.

In this study, our data showed a clear relationship between mRPSMT and the time over which radiological improvement occurs; increase in mRPSMT is associated with a delay in postoperative radiological improvement time. We found no relation between elastin content nor collagen-to-smooth muscle thickness and postoperative improvement course.

Issi and his colleagues studied the effect of histopathology of resected PUJ segment during pyeloplasty on the outcome of surgery; they found no difference in quantity collagen type 3, elastin content, fibrosis, nor Cajal cells between the two study groups; group of immediate postoperative improvement and group of delayed improvement, so they concluded that none of these factors has impact on the outcome of surgery; this is supported by our data regarding elastin content and collagen [10].

In a trail to compare histologically between normal PUJ and PUJO, Doğan et al. studied number of interstitial cells, nerves, presence of fibrosis and inflammation in normal PUJ and PUJO, and they found no difference in these items between the two groups, so they concluded that none of these factors has a role in pathogenesis of PUJO [14]; on the other hand, some authors investigate the relation between the presence of interstitial cells in the resected segment of PUJ during pyeloplasty and the postoperative outcome, and they found that there is no interstitial cells found in the segments resected from cases that had bad surgical outcome [15].

We found statistically significant difference in the mean mRPSMT between improved and non-improved patient at 3, 6 month postoperative; however, this difference was not significant at 12 month and this was supported by Han et al., Kaselas et al. However in their study, they found positive correlation between mRPSMT and delayed postoperative radiographic improvement [3, 9].

Some reports showed increase in elastin content and correlate with delaying in postoperative improvement; this could be due to decrease elasticity and compliance [12, 16]. Kim and his colleagues correlate between collagen-to-smooth muscle tissue matrix ratio in PUJO and the outcome of pyeloplasty; they observed that the lower the tissue matrix ratio, the better improvement of hydronephrosis postoperative [13], whereas we found no significant relation of elastin content nor collagen-to-smooth muscle ratio to postoperative recovery course.

Persistent obstruction associated with prolonged time interval should be a worrying sign for both the parents and the treating surgeon. We can finally conclude from our results that the information retrieved from the histopathological examination of the obstructed PUJ samples after a dismembered pyeloplasty has an important clinical implication to be considered and thus could be used as a prognostic factor. Patient’s postoperative course and expected timing of hydronephrosis improvement, based on the thickness of their renal pelvis smooth muscle, should take much attention by the surgeon during the patient’s postoperative recovery time and until the first postoperative radiographic examination. Taking the histopathological data into consideration could successfully help the surgeon to roughly estimate the expected time over which improvement occur.

The limitation of the study was relatively small number of patients.

Conclusions

Our study concluded that mRPSMT can be used as a significant indicator of postoperative prognosis course, while elastin content and collagen-to-smooth muscle ratio have no role in this; thus, we believe that mRPSMT can provide the surgeon with important prognostic factor which can help in prediction of postoperative course.