Table 1 Genes primers and corresponding annealing temperatures for MSP
From: Exploring urine sediments as a non-invasive method for DNA methylation detection in bladder cancer
Gene |  | Forward (5’ – > 3’) | Reverse (5’ – > 3’) | Product size (pb) | Tm (°C) |
|---|---|---|---|---|---|
TWIST1 | M | CCGTCGCCTTCCTCCGACGA | TGCGTTAGGGTTCGGGGGCGT | 100 | 64 |
U | TTGGATGGGGTTGTTATTGT | CCTAACCCAAACAACCAACC | 79 | 63 | |
hTERT | M | GAGGTATTTCGGGAGGTTTCGC | ACTCCGAACACCACGAATACCG | 121 | 58 |
U | GGGAGGTATTTTGGGAGGTTTTGT’ | CAAACTCCAAACACCACAAATACCA | 126 | 58 | |
NID2 | M | TAGTATTGGTAACGACGATAGTATC | AAATTCGAAACTAACGCGACACG | 141 | 58 |
U | TAGTATTGGTAATGATGATAGTATT | AAATTCAAAACTAACACAACACA | 141 | 55 | |
VIM | M | TTATAAAAATAGCGTTTTCGGC | ATAACGCGAACTAACTCCCG | 143 | 59 |
U | GGGTTATAAAAATAGTGTTTTTGGT | ACAATAACACAAACTAACTCCCA | 149 | 57 |